Journal: Exploration
Article Title: Personalized Cancer Immunotherapy Boosted by cGAS‐STING‐Targeted Nanovaccines in Combination With Nutrient Modulation
doi: 10.1002/EXP.20240183
Figure Lengend Snippet: nECTs combined with FMD for lymph node targeting and stimulation of adaptive immune responses in vivo. (A) Ex vivo IVIS imaging and (B) quantitative analysis of fluorescence intensity in draining lymph nodes after treatment with free ICG or ICG‐labelled BnECTs for 24 h. (C) Schematic illustration of antigen‐specific T cell response stimulation using nECTs with FMD treatment. (D) Flow cytometry data and (E) quantitative analysis of IFN‐γ‐positive cells in CD3 + CD8 + T cells in lymph nodes on Day 22 post‐immunization of BALB/C mice. (F) Quantitative analysis of TNF‐α‐positive cells in CD3 + CD8 + T cells in lymph nodes on Day 22 post‐immunization of BALB/C mice. Quantitative analysis of (G) IFN‐γ, (H) TNF‐α, (I) IL‐6, and (J) IL‐12p70 in the serum of the BALB/C mice with various treatments. Error bars represent ± s.d. ( n = 4). Statistical significance was assessed by one‐way ANOVA with Tukey test. * p < 0.05, ** p < 0.01.
Article Snippet: Subsequently, the gathered lymph nodes underwent scrutiny employing an innovative in vivo optical imaging mechanism, notably the IVIS Spectrum model from PerkinElmer, which facilitated the detection and quantification of the fluorescent emissions from the ICG‐marked components within these nodes and additional organs.
Techniques: In Vivo, Ex Vivo, Imaging, Fluorescence, Flow Cytometry